Thiol-disulfide exchange is involved in the catalytic mechanism of peptide methionine sulfoxide reductase
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چکیده
منابع مشابه
Novel Mechanism for Scavenging of Hypochlorite Involving a Periplasmic Methionine-Rich Peptide and Methionine Sulfoxide Reductase
UNLABELLED Reactive chlorine species (RCS) defense mechanisms are important for bacterial fitness in diverse environments. In addition to the anthropogenic use of RCS in the form of bleach, these compounds are also produced naturally through photochemical reactions of natural organic matter and in vivo by the mammalian immune system in response to invading microorganisms. To gain insight into b...
متن کاملMethionine sulfoxide reductase A is a stereospecific methionine oxidase.
Methionine sulfoxide reductase A (MsrA) catalyzes the reduction of methionine sulfoxide to methionine and is specific for the S epimer of methionine sulfoxide. The enzyme participates in defense against oxidative stresses by reducing methionine sulfoxide residues in proteins back to methionine. Because oxidation of methionine residues is reversible, this covalent modification could also functio...
متن کاملTheoretical insights into the mechanism for thiol/disulfide exchange.
The mechanism for thiol/disulfide exchange has been studied with high-level theoretical calculations. Free energies, transition structures, charge densities, and solvent effects along the reaction pathway have been determined for the first time. Mechanistic results agree with experimental data, and support the idea that the thiolate is the reacting species and that the reaction indeed proceeds ...
متن کاملSelenium and the methionine sulfoxide reductase system.
Selenium is a chemical element participating in the synthesis of selenocysteine residues that play a pivotal role in the enzymatic activity efficiency of selenoproteines. The methionine sulfoxide reductase (Msr) system that reduces methionine sulfoxide (MetO) to methionine comprises the selenoprotein MsrB (MsrB1) and the non-selenoprotein MsrA, which reduce the R- and the S- forms of MetO, resp...
متن کاملPhysical map location of the peptide methionine sulfoxide reductase gene on the Escherichia coli chromosome.
The enzyme peptide methionine sulfoxide reductase (MsrA), the product of the msrA gene, catalyzes the reduction of methionine sulfoxide residues in proteins to methionine (1, 3, 4). During aerobic metabolism, methionine in proteins may be oxidized to methionine sulfoxide by biological reagents such as hydrogen peroxide, hydroxyl radicals, and hypochlorite and superoxide ions (reviewed in refere...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2000
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.97.12.6463